Chromosome-level genome assembly of the two-spotted spider mite Tetranychus urticae

Chromosome-level genome assembly of the two-spotted spider mite Tetranychus urticae

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Abstract The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a notorious pest in agriculture that has developed resistance to almost all chemical types used for its control.Here, we assembled a Brush Roller chromosome-level genome for the TSSM using Illumina, Nanopore, and Hi-C sequencing technologies.The assembled contigs had a total length of 103.

94 Mb with an N50 of 3.46 Mb, with 87.7 Mb of Camisoles 34 contigs anchored to three chromosomes.

The chromosome-level genome assembly had a BUSCO completeness of 94.8%.We identified 15,604 protein-coding genes, with 11,435 genes that could be functionally annotated.

The high-quality genome provides invaluable resources for the genetic and evolutionary study of TSSM.